International Journal of

ADVANCED AND APPLIED SCIENCES

EISSN: 2313-3724, Print ISSN: 2313-626X

Frequency: 12
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 Volume 11, Issue 12 (December 2024), Pages: 174-179

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 Original Research Paper

Potential of endophytic bacteria from Kailan plant roots in suppressing the growth of Sclerotium rolfsii (Sacc.)

 Author(s): 

 Rudi Hartono 1, *, Cheppy Wati 2, Titis Pury Purboningtyas 1, Johanes Amirrullah 3

 Affiliation(s):

 1Sustainable Agricultural Extension Study Program, Bogor Agricultural Development Polytechnic, Bogor, Indonesia
 2Horticulture Agribusiness Study Program, Bogor Agricultural Development Polytechnic, Bogor, Indonesia
 3South Sumatra Agricultural Instrument Standards Implementation Center, Palembang, Indonesia

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 * Corresponding Author. 

  Corresponding author's ORCID profile: https://orcid.org/0000-0002-7053-2753

 Digital Object Identifier (DOI)

 https://doi.org/10.21833/ijaas.2024.12.019

 Abstract

The use of biological agents, such as endophytic bacteria, provides an environmentally friendly and safe method for controlling diseases caused by fungi. Sclerotium rolfsii (Sacc.), a fungus, causes damping-off disease in various horticultural plants, including chili plants. This study aimed to use endophytic bacteria from the roots of Kailan plants to suppress the growth of S. rolfsii. The research involved 1) isolating endophytic bacteria, 2) testing for biosafety, 3) propagating S. rolfsii, and 4) testing the antagonistic effects of the endophytic bacteria. The study found 36 isolates of endophytic bacteria. Bacterial colonies isolated from Kailan roots showed diversity in color and shape, with growth rates observed 48 hours post-isolation. Three isolates tested positive for hemolysis, and six isolates were hypersensitive. The antagonism test indicated that isolates grown on Tryptic soy agar (TSA) media demonstrated greater inhibitory effects on S. rolfsii than those on Potato dextrose agar (PDA) media, with a clearer inhibition zone appearing each day. In total, six isolates of endophytic bacteria from Kailan roots inhibited the growth of S. rolfsii.

 © 2024 The Authors. Published by IASE.

 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

 Keywords

 Endophytic bacteria, Fungal disease control, Sclerotium rolfsii inhibition, Kailan plant roots, Antagonism testing

 Article history

 Received 26 June 2024, Received in revised form 2 November 2024, Accepted 23 November 2024

 Acknowledgment

No Acknowledgment.

 Compliance with ethical standards

 Conflict of interest: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

 Citation:

 Hartono R, Wati C, Purboningtyas TP, and Amirrullah J (2024). Potential of endophytic bacteria from Kailan plant roots in suppressing the growth of Sclerotium rolfsii (Sacc.). International Journal of Advanced and Applied Sciences, 11(12): 174-179

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 Figures

 Fig. 1 Fig. 2 Fig. 3 

 Tables

 Table 1

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