International journal of

ADVANCED AND APPLIED SCIENCES

EISSN: 2313-3724, Print ISSN:2313-626X

Frequency: 12
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 Volume 6, Issue 12 (December 2019), Pages: 58-66

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 Original Research Paper

 Title: Injectable collagen-chitosan hydrogel using ultrasonic pretreated ovine tendon collagen

 Author(s): Lau Sin Mun 1, Masrina Mohd Nadzir 1, *, Shiplu Roy Chowdhury 2, Mohd Fauzi Mh Busra 2, Azlina Harun Kamaruddin 1, Gong Wee Jie 1

 Affiliation(s):

 1School of Chemical Engineering, Universiti Sains Malaysia, George Town, Malaysia
 2Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia

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 * Corresponding Author. 

  Corresponding author's ORCID profile: https://orcid.org/0000-0003-4530-5863

 Digital Object Identifier: 

 https://doi.org/10.21833/ijaas.2019.12.006

 Abstract:

Normally discarded as waste, ovine tendon collagen has great potential for use as an injectable hydrogel. Typical collagen used for biomedical applications is derived from epidermal tissue. Compared to the epidermal collagen, tendon collagen consists of collagen fibrils with large diameters. This limits the conjugation of phenolic hydroxyl (Ph) groups to tendon collagen for the synthesis of an injectable hydrogel. In this study, pretreatment process by ultrasonication was used to prevent the aggregation of ovine tendon collagen fibril, thus reducing fibril diameters and increasing the surface area for effective conjugation of Ph groups to collagen-chitosan (Col-Chit) composite. In situ gelation of Col-Chit-Ph composite was achieved via peroxidase-catalyzed crosslinking reaction at physiological conditions. The collagen to chitosan (Col: Chit) ratio was found to significantly influence the physical, mechanical and biological properties of hydrogels. The higher composition of chitosan in the hydrogel with 1:1 Col: Chit ratio resulted in the shortest gelation time (< 1 minute) and higher mechanical strength (> 0.35 N) in all conditions. However, the poor cell growth rate of hydrogel at this Col: Chit ratio might limit its further use. The hydrogel with 3:2 Col: Chit ratio was mechanically stable and has the highest cell growth rate among others with difference of cell growth rate of about 94 % compared to the 1:1 ratio. Taking into account these biological features, hydrogel with 3:2 Col: Chit ratio is suggested for potential use in biomedical applications. This study shows the feasibility of using ultrasonic pretreatment method for collagen with large fibril diameter. 

 © 2019 The Authors. Published by IASE.

 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

 Keywords: Collagen, Chitosan, Hydrogel, Ultrasonication

 Article History: Received 1 July 2019, Received in revised form 30 September 2019, Accepted 1 October 2019

 Acknowledgement:

This study was funded by the Bridging-Insentif Grant (304/PJKIMIA/6316445) from Universiti Sains Malaysia and the Fundamental Research Grant Scheme (203/PJKIMIA/6071379) from Ministry of Higher Education, Malaysia.

 Compliance with ethical standards

 Conflict of interest:  The authors declare that they have no conflict of interest.

 Citation:

 Mun LS, Nadzir MM, and Chowdhury SR et al. (2019). Injectable collagen-chitosan hydrogel using ultrasonic pretreated ovine tendon collagen. International Journal of Advanced and Applied Sciences, 6(12): 58-66

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 Figures

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 Tables

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