Volume 6, Issue 1 (January 2019), Pages: 24-28

----------------------------------------------

 Original Research Paper

 Title: Extracts from egg strings of sea hare (Dolabella auricularia): Yield, antioxidant activity, zoochemical profile, and toxicity

 Author(s): Janeth C. Tayone ^{1, 2,} *, Romeo M. Del Rosario ², Oliva P. Canencia ²

 Affiliation(s):

 ¹Institute of Agriculture and Life Sciences, Davao Oriental State College of Science and Technology, Mati, 8200, Davao Oriental, Philippines
 ²College of Science and Technology Education, University of Science and Technology of Southern Philippines, Lapasan, Cagayan de Oro, 9000, Philippines

  Full Text - PDF          XML

 * Corresponding Author. 

  Corresponding author's ORCID profile: https://orcid.org/0000-0003-0109-4793

 Digital Object Identifier: 

 https://doi.org/10.21833/ijaas.2019.01.003

 Abstract:

This study aimed to evaluate the crude extract yield, secondary metabolites, antioxidant and cytotoxic activities of the different crude extracts of egg strings from sea hare (Dolabella auricularia) taken from Guang-guang, Pujada Bay, Davao Oriental. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method showed that the antioxidant activity of the crude extract of ethanol was greater than ethyl acetate and hexane in a dose-dependent manner. Ethanolic extract with a yield of 28.96 mg/g showed the highest percentage inhibition of 24.86% at 1000 µg/mL. The antioxidant activity may be attributed to known antioxidant metabolites, especially the phenolic substances, and some forms of synergism. The result also revealed the presence of alkaloids, saponins, steroids, tannins, and terpeneoids. On the other hand, the brine shrimp assay for all extracts gave LC₅₀ > 1,000 µg/mL which indicated the absence of potential cytotoxic substances. The result of this study increases the nutritional potential values and provide baseline information for another possible source of future novel antioxidants that can be used in food and pharmaceutical industries. However, further investigations must be conducted to explore other biological activities of the extracts. 

 © 2018 The Authors. Published by IASE.

 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

 Keywords: Antioxidant, Cytotoxic, Sea hare, Zoochemical

 Article History: Received 6 July 2018, Received in revised form 2 November 2018, Accepted 2 November 2018

 Acknowledgement:

The authors would like to acknowledge the Research, Development and Extension Division of Davao Oriental State College of Science and Technology for providing the funds for this study.

 Compliance with ethical standards

 Conflict of interest:  The authors declare that they have no conflict of interest.

 Citation:

 Tayone JC, Rosario RMD, and Canencia OP (2019). Extracts from egg strings of sea hare (Dolabella auricularia): Yield, antioxidant activity, zoochemical profile, and toxicity. International Journal of Advanced and Applied Sciences, 6(1): 24-28

 Permanent Link to this page

 Figures

 Fig. 1 Fig. 2

 Tables

 Table 1 Table 2 Table 3

----------------------------------------------

 References (19) 

1. Adedapo A, Jimoh F, and Afolayan A (2011).  Comparison of the nutritive value and biological activities of the acetone, methanol and water extracts of the leaves of Bidens pilosa and Chenopodium album.  Acta Poloniae Pharmaceutica - Drug Research, 68(1): 83-92.   [Google Scholar]
2. Adedapo AA, Jimoh FO, Koduru S, Afolayan AJ, and Masika PJ (2008). Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea. BMC Complementary and Alternative Medicine, 8(1): 53-61. https://doi.org/10.1186/1472-6882-8-53    [Google Scholar] PMid:18803865 PMCid:PMC2556645
3. Alam MN, Bristi NJ, and Rafiquzzaman M (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 21(2): 143-152. https://doi.org/10.1016/j.jsps.2012.05.002    [Google Scholar]  PMid:24936134 PMCid:PMC4052538
4. Anokwuru CP, Anyasor GN, Ajibaye O, Fakoya O, and Okebugwu P (2011). Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three Nigerian medicinal plants. Nature and Science, 9(7): 53-61.    [Google Scholar]
5. Chojnacka K, Saeid A, Witkowska Z, and Tuhy L (2012). Biologically active compounds in seaweed extracts—the prospects for the application. The Open Conference Proceedings Journal, 3(1): 20-28. https://doi.org/10.2174/1876326X01203020020    [Google Scholar]
6. Dhanani T, Shah S, Gajbhiye NA, and Kumar S (2017). Effect of extraction methods on yield, phytochemical constituents and antioxidant activity of Withania somnifera. Arabian Journal of Chemistry, 10(1): S1193-S1199. https://doi.org/10.1016/j.arabjc.2013.02.015    [Google Scholar]
7. Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, and Ju YH (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3): 296-302. https://doi.org/10.1016/j.jfda.2013.11.001    [Google Scholar]  PMid:28911418
8. Guevara BQ (2005). A guidebook to plant screening: phytochemical and biological. University of Santo Tomas Publishing House, Manila, Philippines.    [Google Scholar]
9. Kawsar S, Aftabuddin S, Yasumitsu H, and Ozeki Y (2010). The cytotoxic activity of two D-galactose-binding lectins purified from marine invertebrates. Archives of Biological Sciences, 62(4): 1027-1034. https://doi.org/10.2298/ABS1004027K    [Google Scholar]
10. Krishnaraju AV, Rao TV, Sundararaju D, Vanisree M, Tsay HS, and Subbaraju GV (2005). Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay. International Journal of Applied Science and Engineering, 3(2): 125-34.    [Google Scholar]
11. Lozada PWM, Flores LAJ, Tan RM, and Dy DT (2005). Abundance and ingestion rate of the sea hare, Dolabella auricularia (Lightfoot 1786) in a shallow embayment (Eastern Mactan Is., Cebu, Central Philippines). Philippine Scientist, 42: 67-78.    [Google Scholar]
12. Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, and McLaughlin JL (1982). Brine shrimp: a convenient general bioassay for active plant constituents. Planta Medica, 45(5): 31-34. https://doi.org/10.1055/s-2007-971236    [Google Scholar]
13. Pallab K, Tapan BK, Pal TK, and Kalita R (2013). Estimation of total flavonoids content (TFC) and anti oxidant activities of methanolic whole plant extract of Biophytum sensitivum Linn. Journal of Drug Delivery and Therapeutics, 3(4): 33-37.    [Google Scholar]
14. Pennings SC, Nadeau MT, and Paul VJ (1993). Selectivity and growth of the generalist herbivore Dolabella auricularia feeding upon complementary resources. Ecology, 74(3): 879-890. https://doi.org/10.2307/1940813    [Google Scholar]
15. Pepito AR, Delan GG, Asakawa M, Ami LJ, Yap EES, Olympia MS, and Lamayo MHA (2015). Nutritional quality of the egg mass locally known as “lukot’of the wedge seahare dolabella auricularia (Lightfoot, 1786). Tropical Technology Journal, 1(19): 1-6.    [Google Scholar]
16. Pereira DM, Valentão P, Pereira JA, and Andrade PB (2009). Phenolics: From chemistry to biology. Molecules, 14(6): 2202-2211. https://doi.org/10.3390/molecules14062202    [Google Scholar]
17. Saeed N, Khan MR, and Shabbir M (2012). Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complementary and Alternative Medicine, 12(1): 221-233. https://doi.org/10.1186/1472-6882-12-221    [Google Scholar]  PMid:23153304 PMCid:PMC3524761
18. Simmons TL, Andrianasolo E, McPhail K, Flatt P, and Gerwick WH (2005). Marine natural products as anticancer drugs. Molecular Cancer Therapeutics, 4(2): 333-342.    [Google Scholar] PMid:15713904
19. Sreejamole KL and Radhakrishnan CK (2010). Preliminary qualitative chemical evaluation of the extracts from mussel Perna viridis. International Journal of Pharmaceutical Sciences Review and Research, 5(2): 38-42.    [Google Scholar]