Fig. 1: Sea hare (A) and its egg strings (B) found in Guang-guang, Pujada Bay, Davao Oriental

Table 1: The crude extract yield of egg strings in different solvents

Crude Extract Yield (mg/g)	Crude Extract Concentration (mg/mL)	Crude Extract
28.96 ± 0.25	2.73 ±0.02	Ethanol
29.21 ± 0.87	2.76±0.08	Ethyl acetate
18.38 ± 0.35	1.74±0.03	Hexane

Each value is the average of three measurements; ±: standard deviation

Fig. 2: The percentage inhibition of the three different crude extracts of egg strings from sea hare

Table 2: Zoochemical analysis of egg strings in different crude extracts

Hexane	Ethyl Acetate	Ethanol	Zoochemicals
-	-	+	Alkaloids
-	-	-	Anthraquinones
-	-	-	Coumarins
-	-	-	Flavonoids
-	-	-	Glycosides
+	+	+	Phenols
-	-	-	Quinones
+++	++	+	Saponins
+++	++	+	Steroids
+	++	-	Tannins
+++	++	+	Terpenoids

(-): absence; (+): trace amount; (++): moderate; (+++): abundant

Each trial was done in three replicates

Table 3: Effects of crude extracts from egg strings on brine shrimp lethality assay

%Mortality	Total Number of Survivors	Concentration (µg/mL)	Crude Extracts
0	30	0	Control
0 0 3.33	30 30 29	10 100 1000	Ethanol
0 0 3.33	30 30 29	10 100 1000	Ethyl acetate
0 0 3.33	30 30 29	10 100 1000	Hexane

Values are the mean of three replicates