Phytochemical screening, cytotoxicity, mutagenicity, antimutagenicity, and protective potentials of the different solvent extracts from the air-dried leaves of Crescentia cujete Linn.

Billacura, Merell P.; Pangcoga, AKelvin Kiram J.

International Journal of Advanced and Applied Sciences

Int. j. adv. appl. sci.

EISSN: 2313-3724

Print ISSN: 2313-626X

Volume 4, Issue 4 (April 2017), Pages: 118-126

Title: Phytochemical screening, cytotoxicity, mutagenicity, antimutagenicity, and protective potentials of the different solvent extracts from the air-dried leaves of Crescentia cujete Linn.

Author(s): Merell P. Billacura ^{1, 2,} *, Kelvin Kiram J. Pangcoga ¹

Affiliation(s):

¹Chemistry Department, College of Natural Sciences and Mathematics, Mindanao State University-Main Campus, Marawi City, Lanao del Sur 9700, Philippines
²Mamitua Saber Research and Technology Center, Mindanao State University-Main Campus, Marawi City, Lanao del Sur 9700, Philippines

https://doi.org/10.21833/ijaas.2017.04.017

Full Text - PDF XML

Abstract:

Crescentia cujete Linn. is a fast-growing evergreen tree in the Philippines. Folklore says C. cujete Linn. has been used as an alternative medicine to treat ailments such as hematoma and tumors. In this study, air-dried leaves of C. cujete Linn. was prepared and partitioned using hexane, and ethyl acetate as solvents and was qualitatively assessed for the presence of phytochemical constituents. Secondary metabolites like flavonoids, tannins, reducing sugar, and steroids were observed in the preliminary phytochemical screening of hexane, ethyl acetate and aqueous extracts. Cytotoxicity of the different extracts were determined using brine shrimp lethality test (BSLT). Median lethal concentration (LC₅₀) of the hexane, aqueous, and crude ethanolic extracts were determined after 6 hours of exposure showing 572, 3048 and 220 ppm, respectively. Moreover, LC₅₀ of the hexane, aqueous, and crude ethanolic extract, after 24 hours of exposure, were also determined showing 0, 0.184, and 6.47 ppm, respectively. The hexane, aqueous, and crude ethanolic extracts of C. cujete Linn. were also tested for its mutagenicity, antimutagenicity, and protective potentials using peripheral blood micronucleus test. The number of micronucleated polychromatic erythrocyte (MNPCE) observed from the mutagenicity test of hexane, aqueous, and crude ethanolic extract administered at a dose of 50mg/kg and 100mg/kg showed an average mean of 16.7,14.6 and 14.7, respectively, are lower compared to that of the positive control, methyl methanesulfonate (MMS), showing 49.5 number of MNPCE. Also, results for the antimutagenicity and protective potentials of hexane, aqueous, and crude ethanolic extract administered at a dose of 50mg/kg and 100mg reduced the number of MNPCEs produced by the MMS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Keywords: Miracle fruit, Crescentia cujete, Artemia salina

Article History: Received 10 January 2017, Received in revised form 24 March 2017, Accepted 30 March 2017

Digital Object Identifier:

https://doi.org/10.21833/ijaas.2017.04.017

Citation:

Billacura MP and Pangcoga KKJ (2017). Phytochemical screening, cytotoxicity, mutagenicity, antimutagenicity, and protective potentials of the different solvent extracts from the air-dried leaves of Crescentia cujete Linn.. International Journal of Advanced and Applied Sciences, 4(4): 118-126

http://www.science-gate.com/IJAAS/V4I4/Billacura.html

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