International journal of

ADVANCED AND APPLIED SCIENCES

EISSN: 2313-3724, Print ISSN:2313-626X

Frequency: 12
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 Volume 4, Issue 12 (December 2017), Pages: 31-35

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 Original Research Paper

 Title: Tertiary structure prediction of bromelain from Ananas Comosus using comparative modelling method

 Author(s):  Fatahiya Mohamed Tap 1, Fadzilah Adibah Abd Majid 2, *, Nurul Bahiyah Ahmad Khairudin 1

 Affiliation(s):

 1Chemical Energy Conversion and Applications Group, Malaysia Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia
 2Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Malaysia

 https://doi.org/10.21833/ijaas.2017.012.007

 Full Text - PDF          XML

 Abstract:

Bromelain is a general name for a family of sulfhydryl which can be found in the proteolytic enzyme group from pineapples (Ananas Comosus).  This study focuses on the prediction of three dimensional structures (3D) of stem bromelain using the method of comparative modelling. The amino acid sequence of bromelain was obtained from the NCBI database was used as a tool to search for proteins with known 3D structures related to the target sequence. Suitable template was chosen based on >30% sequence similarity and lowest e-value. Based on these criteria, 1YAL was selected as the best template with 55% of sequence similarity and 9x10-52 of e-value. 

 © 2017 The Authors. Published by IASE.

 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

 Keywords: Bromelain, Comparative modelling, Sequence alignment

 Article History: Received 21 February 2017, Received in revised form 2 October 2017, Accepted 8 October 2017

 Digital Object Identifier: 

 https://doi.org/10.21833/ijaas.2017.012.007

 Citation:

 Tap FM, Majid FAA, Khairudin NBA (2017). Tertiary structure prediction of bromelain from Ananas Comosus using comparative modelling method. International Journal of Advanced and Applied Sciences, 4(12): 31-35

 Permanent Link:

 http://www.science-gate.com/IJAAS/V4I12/Tap.html

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