International Journal of

ADVANCED AND APPLIED SCIENCES

EISSN: 2313-3724, Print ISSN: 2313-626X

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 Volume 10, Issue 7 (July 2023), Pages: 211-218

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 Original Research Paper

Evaluation of Tribulus terrestris effect on kidney and liver function in healthy mice

 Author(s): 

 Omaima Nasir 1, *, Omaima Ahmed 2, Roua S. Baty 3, Saad H. Alotaibi 4, Nada Alqadri 1, Suzan A. Abushal 5, Salam Elsayed 6, Hind Toufig 7

 Affiliation(s):

 1Department of Biology, Turabah University College, Taif University, Taif, Saudi Arabia
 2Department of Biology, University College of Taymaa, University of Tabuk, Tabuk, Saudi Arabia
 3Department of Biotechnology, College of Sciences, Taif University, Taif, Saudi Arabia
 4Department of Chemistry, Turabah University College, Taif University, Taif, Saudi Arabia
 5Program of Food Sciences and Nutrition, Turabah University College, Taif University, Taif, Saudi Arabia
 6Department of Clinical Laboratory Sciences, Turabah University College, Taif University, Taif, Saudi Arabia
 7Department of Surgery, College of Medicine, King Faisal University, Alahsa, Saudi Arabia

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 * Corresponding Author. 

  Corresponding author's ORCID profile: https://orcid.org/0000-0002-3021-6473

 Digital Object Identifier: 

 https://doi.org/10.21833/ijaas.2023.07.023

 Abstract:

This research aims to evaluate the pharmacological efficacy of aqueous spine extracts of Tribulus terrestris (TT) on liver and kidney function through physiological, biochemical, haematological, and histological assessments. Tribulus terrestris is renowned for its therapeutic benefits in traditional medicine practices in China, India, Pakistan, and Sudan for treating various chronic ailments. The study employed 5 weight per volume percentage (% w/v) of TT aqueous spines extract. BALB/c mice, both male and female, aged 4-6 weeks, were maintained in controlled ecological conditions (temperature: 22-24°C, humidity: 50-70%, light/dark cycle: 12-h). The mice were divided into two groups: the control group received normal tap water throughout the experimental period, while the other group was administered 5% (w/v) TT spines solution for 15 days. The outcomes of BALB/c mice treated with 5% Tribulus terrestris were compared with the control group after 15 days, revealing no significant difference in the drug's efficacy. The physiological results indicated reduced body weight, food intake, and adverse effects in the biochemical profile, including urinary and serum electrolyte levels, liver function tests, increased random blood glucosem, aspartate aminotransferase (AST), alkaline phosphatase (ALP), alanine aminotransferase (ALT), bilirubin (BIL), cholesterol (CHO), high-density lipoprotein (HDL), and triglycerides (TRI). Similarly, kidney function tests showed increased Urinary Nitrogen and creatinine plasma levels, with low blood urea nitrogen (BUN), urinary creatinine, and glomerular filtration rate (GFR). However, no significant difference was observed in haematological profile readings. In conclusion, the findings suggest that the aqueous spine extract of Tribulus terrestris does not demonstrate beneficial effects on liver and kidney function, indicating that this study supports the notion that the plant cannot be utilized as a medicinal remedy.

 © 2023 The Authors. Published by IASE.

 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

 Keywords: Tribulus terrestris, Pharmacological efficacy, Liver and kidney function, BALB/c mice

 Article History: Received 15 February 2022, Received in revised form 19 May 2023, Accepted 11 June 2023

 Acknowledgment 

We acknowledge Taif University Researchers Supporting Project number (TURSP-2020/269), Taif University, Kingdom of Saudi Arabia.

 Funding

We thank Taif University Researchers Supporting Project number (TURSP-2020/269), Taif University, Taif, Saudi Arabia. This work was supported by Taif University Research Supporting Project number (TURSP-2020/269), Taif University, Taif, Saudi Arabia.

 Compliance with ethical standards

 Institutional review board statement

All procedures carried out in the study containing animals were in accordance with the ethical standards of the Ethical Committee of Deanship of Taif University. All mice were sacrificed under anaesthesia; efforts were made to reduce pain and suffering to the mice, and with the Helsinki Declaration of 1964 and its later amendments or comparable ethical standards.

 Conflict of interest: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

 Citation:

 Nasir O, Ahmed O, Baty RS, Alotaibi SH, Alqadri N, Abushal SA, Elsayed S, and Toufig H (2023). Evaluation of Tribulus terrestris effect on kidney and liver function in healthy mice. International Journal of Advanced and Applied Sciences, 10(7): 211-218

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 Figures

 Fig. 1 Fig. 2 Fig. 3

 Tables

 Table 1 

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