International Journal of

ADVANCED AND APPLIED SCIENCES

EISSN: 2313-3724, Print ISSN: 2313-626X

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 Volume 10, Issue 2 (February 2023), Pages: 175-181

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 Original Research Paper

 Histomorphological study of the effect of mint on the uterus and ovary of adult Wistar rats

 Author(s): 

 Arpita Soy, Jeneeta Baa *, Dibya Prabha Bara

 Affiliation(s):

 Department of Anatomy, Veer Surendra Sai Institute of Medical Sciences and Research, Burla, India

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 * Corresponding Author. 

  Corresponding author's ORCID profile: https://orcid.org/0000-0002-1618-3580

 Digital Object Identifier: 

 https://doi.org/10.21833/ijaas.2023.02.021

 Abstract:

Mentha piperita is a medicinal plant used for treating human diseases for thousands of years. However, the traditional medicinal usage of herbs by humans is imperfect and unscientific by modern standards. It is the result of countless trial-and-error tests that people have conducted, and so traditional usage points the way to natural therapeutic usage. "Natural" does not necessarily mean "safe." This study aimed to evaluate the effects of ethanolic extract of Mentha piperita leaves on histo-morphological changes in the ovary and uterus of adult female Wistar rats. Hence this study was undertaken to evaluate its impact on the gonadal organ in female Wistar albino rats in 18 female albino rats of age 6-8 weeks which were divided into 3 groups. Group I was treated as control, Group II and III received orally 200mg/kg and 400mg/kg of ethanolic extract of Mentha piperita respectively for 60 days. Then the rats were sacrificed and the ovaries and uterus were processed for haematoxylin and eosin staining to study the effect on the estrous phase, ovulation, and implantation. The ovarian germinal epithelium was found to be flattened at places and disrupted. The stroma shows more fibrosis with severe degeneration. Granulosa cells are also disrupted. Follicles show features of degeneration. Degenerating oocytes are also seen in places. The endometrium lining epithelium is disrupted, vascularity was increased and the myometrium has become edematous. It is shown in previous studies that Mentha arvensis, Mentha spicate (spearmint), and Mentha piperita have got adverse effects on the reproductive system but in our study on female fertility, we found that Mentha piperita showed increased folliculogenesis and many degenerated follicles at different stages of development.

 © 2022 The Authors. Published by IASE.

 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

 Keywords: Mentha piperita, Wister rats, Estrous, Fertility

 Article History: Received 11 July 2022, Received in revised form 9 November 2022, Accepted 10 November 2022

 Acknowledgment 

No Acknowledgment.

 Compliance with ethical standards

 Ethical consideration

IAEC-approved experimental protocol and care of animals were taken as per CPCSEA guidelines, Dept. of Animal Welfare, Government of India.

 Conflict of interest: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

 Citation:

 Soy A, Baa J, and Bara DP (2023). Histomorphological study of the effect of mint on the uterus and ovary of adult Wistar rats. International Journal of Advanced and Applied Sciences, 10(2): 175-181

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 Figures

 Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6

 Tables

 Table 1 Table 2 Table 3 Table 4 

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 References (14)

  1. Abasian Z, Rostamzadeh A, Mohammadi M, Hosseini M, and Rafieian-Kopaei M (2018). A review on role of medicinal plants in polycystic ovarian syndrome: pathophysiology, neuroendocrine signaling, therapeutic status and future prospects. Middle East Fertility Society Journal, 23(4): 255-262. https://doi.org/10.1016/j.mefs.2018.04.005   [Google Scholar]
  2. Ali AA, El-Seify GH, El Haroun HM, and Soliman MA (2014). Effect of monosodium glutamate on the ovaries of adult female albino rats and the possible protective role of green tea. Menoufia Medical Journal, 27(4): 793-800. https://doi.org/10.4103/1110-2098.149773   [Google Scholar]
  3. Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, and Koirala N (2019). Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal. Plants, 8(4): 96. https://doi.org/10.3390/plants8040096   [Google Scholar] PMid:30978964 PMCid:PMC6524357
  4. Fleming T (1998). PDR for herbal medicines. Medical Economics Company, Montvale, USA.   [Google Scholar]
  5. Grant P (2010). Spearmint herbal tea has significant anti‐androgen effects in polycystic ovarian syndrome. A randomized controlled trial. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 24(2): 186-188. https://doi.org/10.1002/ptr.2900   [Google Scholar] PMid:19585478
  6. Krinke GJ (2000). The laboratory rat: Handbook of experimental animals. Academic Press, Cambridge, UK.   [Google Scholar]
  7. Maurya R, Srivastava S, Kulshreshta DK, and Gupta CM (2004). Traditional remedies for fertility regulation. Current Medicinal Chemistry, 11(11): 1431-1450. https://doi.org/10.2174/0929867043365215   [Google Scholar] PMid:15180576
  8. Mokaberinejad R, Zafarghandi N, Bioos S, Dabaghian FH, Naseri M, Kamalinejad M, and Hamiditabar M (2012). Mentha longifolia syrup in secondary amenorrhea: A double-blind, placebo-controlled, randomized trials. DARU Journal of Pharmaceutical Sciences, 20(1): 1-8. https://doi.org/10.1186/2008-2231-20-97   [Google Scholar] PMid:23351184 PMCid:PMC3556020
  9. Nasri H (2013). Toxicity and safety of medicinal plants. Journal of HerbMed Pharmacology, 2(2): 21-22.   [Google Scholar]
  10. Rita P and Animesh DK (2011). An updated overview on peppermint (Mentha piperita L.). International Research Journal of Pharmacy, 2(8): 1-10.   [Google Scholar]
  11. Singh R, Shushni MA, and Belkheir A (2015). Antibacterial and antioxidant activities of Mentha piperita L. Arabian Journal of Chemistry, 8(3): 322-328. https://doi.org/10.1016/j.arabjc.2011.01.019   [Google Scholar]
  12. Singh SP (1990). Fertility control of female through Sesbania sesban seeds. The Journal of Research and Education in Indian Medicine, 9(4): 27-32.   [Google Scholar]
  13. Spiteri SS (2011). Herbal medicines: Adverse effects and drug-herb interactions. Journal of the Malta College of Pharmacy Practice, (17): 38-42.   [Google Scholar]
  14. Tyler VE (1992). The honest herbal: A sensible guide to the use of herbs and related remedies. Taylor and Francis, Abingdon, USA.   [Google Scholar]