International Journal of

ADVANCED AND APPLIED SCIENCES

EISSN: 2313-3724, Print ISSN: 2313-626X

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 Volume 10, Issue 2 (February 2023), Pages: 156-165

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 Original Research Paper

Effects of Gum Arabic and its nanoparticles on hepato-renal toxicity induced by bromobenzene in male rats: Physiological, histological, and immunological studies

 Author(s): 

 Turki M. Al-Shaikh 1, 2, *

 Affiliation(s):

 1Department of Biological Sciences, Faculty of Science, Northern Border University, Arar, Saudi Arabia
 2Department of Biology, College of Science and Arts at Khulis, University of Jeddah, Jeddah, Saudi Arabia

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 * Corresponding Author. 

  Corresponding author's ORCID profile: https://orcid.org/0000-0002-4469-8581

 Digital Object Identifier: 

 https://doi.org/10.21833/ijaas.2023.02.019

 Abstract:

This experimental study investigates the possible protective effects of Gum Arabic (GA) and its nanoparticles in hepato- and reno-toxicity induced by bromobenzene (BB) in rats and possible mechanisms of action. Thirty-five adult male albino rats were sorted into the following: Group 1 (control), Group 2 (NPs), Group 3 (GA, received 2 ml/kg of 10%w/v aqueous suspension), Group 4 (Gum-NPs, received GA loaded NPs), Group 5 (BB), received 460 mg/kg, Group 6 (GA+BB) and Group 7 (GA-NPs+BB). Treatment was via oral gavage daily for 10 days. Liver and kidney functions were measured in sera and total antioxidant capacity (TAC) was measured in tissue homogenates, and renal and hepatic tissues expression of caspase-3 were immuno-histochemical assessed beside histological alteration using a light microscope. BB treatment produced impairment of liver and kidney functions and decreased TAC activities and increased caspase-3 expressions in the liver and kidney and altered liver and kidney structures. Co-administration of GA and GA loaded on NPs for 10 days alleviated damaged effects of BB, especially in GA-NPs groups in liver and kidney functions and structures, and decrease expression of caspase-3 in the tissues. In conclusion, GA and its NPs had protective actions versus BB-induced destruction of the kidney and liver due to its antioxidant, anti-inflammatory and anti-apoptosis actions.

 © 2022 The Authors. Published by IASE.

 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

 Keywords: Antioxidant, Bromobenzene, Caspase-3, Gum Arabic, Kidney, Liver, Nanoparticles

 Article History: Received 24 May 2022, Received in revised form 24 October 2022, Accepted 6 November 2022

 Acknowledgment 

This author thanks Northern Border University for funding this research. Acknowledgment also was offered to the Nanotechnology unit, Assuit university, and Prof. Soad Shaker; histopathologist KAU, and Assuit University's faculties of medicine for formulation and characterization of nanoparticles besides reviewing the manuscript.

 Compliance with ethical standards

 Ethics approval 

This study was conducted in accordance with ARRIVE Guideline f of Animal and approved by the Ethics Committee of King Abdelaziz University (KAU) (Approval#300-19).

 Conflict of interest: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

 Citation:

 Al-Shaikh TM (2023). Effects of Gum Arabic and its nanoparticles on hepato-renal toxicity induced by bromobenzene in male rats: Physiological, histological, and immunological studies. International Journal of Advanced and Applied Sciences, 10(2): 156-165

 Permanent Link to this page

 Figures

 Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 

 Tables

 Table 1 Table 2 Table 3 

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 References (32)

  1. Akkara PJ and Sabina EP (2020). Pre-treatment with beta carotene gives protection against nephrotoxicity induced by bromobenzene via modulation of antioxidant system, pro-inflammatory cytokines and pro-apoptotic factors. Applied Biochemistry and Biotechnology, 190(2): 616-633. https://doi.org/10.1007/s12010-019-03111-0   [Google Scholar] PMid:31407161
  2. Ali BH, Al-Qarawi AA, Haroun EM, and Mousa HM (2003). The effect of treatment with gum arabic on gentamicin nephrotoxicity in rats: A preliminary study. Renal Failure, 25(1): 15-20.  https://doi.org/10.1081/JDI-120017439   [Google Scholar] PMid:12617329
  3. Ali BH, Ziada A, Al Husseni I, Beegam S, and Nemmar A (2011). Motor and behavioral changes in rats with adenine-induced chronic renal failure: Influence of acacia gum treatment. Experimental Biology and Medicine, 236(1): 107-112. https://doi.org/10.1258/ebm.2010.010163   [Google Scholar] PMid:21239740
  4. Al-Kenanny ER, Al-Hayaly LK, and Al-Badrany AG (2012). Protective effect of Arabic gum on liver injury experimentally induced by gentamycin in mice. Kufa Journal for Veterinary Medical Sciences, 3(1): 174-189.   [Google Scholar]
  5. Ayaz NO, Ramadan KS, Farid HE, and Alnahdi HS (2017). Protective role and antioxidant activity of Arabic gum against trichloroacetate-induced toxicity in liver of male rats. Indian Journal of Animal Research, 51(2): 303-309. https://doi.org/10.18805/ijar.10976   [Google Scholar]
  6. Bashir M and Haripriya S (2016). Assessment of physical and structural characteristics of almond gum. International Journal of Biological Macromolecules, 93: 476-482. https://doi.org/10.1016/j.ijbiomac.2016.09.009   [Google Scholar] PMid:27608543
  7. Calame W, Weseler AR, Viebke C, Flynn C, and Siemensma AD (2008). Gum arabic establishes prebiotic functionality in healthy human volunteers in a dose-dependent manner. British Journal of Nutrition, 100(6): 1269-1275. https://doi.org/10.1017/S0007114508981447   [Google Scholar] PMid:18466655
  8. El-Sharaky AS, Newairy AA, Kamel MA, and Eweda SM (2009). Protective effect of ginger extract against bromobenzene-induced hepatotoxicity in male rats. Food and Chemical Toxicology, 47(7): 1584-1590. https://doi.org/10.1016/j.fct.2009.04.005   [Google Scholar] PMid:19371770
  9. Emerich DF and Thanos CG (2003). Nanotechnology and medicine. Expert Opinion on Biological Therapy, 3(4): 655-663. https://doi.org/10.1517/14712598.3.4.655   [Google Scholar] PMid:12831370
  10. Gopi S and Setty OH (2010). Beneficial effect of the administration of Hemidesmus indicus against bromobenzene induced oxidative stress in rat liver mitochondria. Journal of Ethnopharmacology, 127(1): 200-203. https://doi.org/10.1016/j.jep.2009.09.043   [Google Scholar] PMid:19799985
  11. Hamed MA, El-Rigal NS, and Ali SA (2013). Effects of black seed oil on resolution of hepato-renal toxicity induced bybromobenzene in rats. European Review for Medical and Pharmacological Sciences, 17(5): 569-581.   [Google Scholar]
  12. Isobe N, Sagawa N, Ono Y, Fujisawa S, Kimura S, Kinoshita K, and Deguchi S (2020). Primary structure of gum arabic and its dynamics at oil/water interface. Carbohydrate Polymers, 249: 116843. https://doi.org/10.1016/j.carbpol.2020.116843   [Google Scholar] PMid:32933685
  13. Khan RA, Khan MR, Sahreen S, and Bokhari J (2010). Prevention of CCl4-induced nephrotoxicity with Sonchus asper in rat. Food and Chemical Toxicology, 48(8-9): 2469-2476. https://doi.org/10.1016/j.fct.2010.06.016   [Google Scholar] PMid:20550952
  14. Laurent S, Saei AA, Behzadi S, Panahifar A, and Mahmoudi M (2014). Superparamagnetic iron oxide nanoparticles for delivery of therapeutic agents: Opportunities and challenges. Expert Opinion on Drug Delivery, 11(9): 1449-1470. https://doi.org/10.1517/17425247.2014.924501   [Google Scholar] PMid:24870351
  15. McNeil SE (2005). Nanotechnology for the biologist. Journal of Leukocyte Biology, 78(3): 585-594. https://doi.org/10.1189/jlb.0205074   [Google Scholar] PMid:15923216
  16. Moretti M and Marchioni CF (2007). An overview of erdosteine antioxidant activity in experimental research. Pharmacological Research, 55(4): 249-254. https://doi.org/10.1016/j.phrs.2006.12.006   [Google Scholar] PMid:17267240
  17. Nasir O (2013). Renal and extra renal effects of gum arabic (Acacia senegal)-What can be learned from animal experiments? Kidney and Blood Pressure Research, 37(4-5): 269-279. https://doi.org/10.1159/000350152   [Google Scholar] PMid:24022265
  18. Nasir O, Wang K, Föller M, Bhandaru M, Sandulache D, Artunc F, and Lang F (2010). Downregulation of angiogenin transcript levels and inhibition of colonic carcinoma by gum arabic (Acacia senegal). Nutrition and Cancer, 62(6): 802-810. https://doi.org/10.1080/01635581003605920   [Google Scholar] PMid:20661830
  19. Pannala VR, Estes SK, Rahim M, Trenary I, O’Brien TP, Shiota C, and Wallqvist A (2020). Mechanism-based identification of plasma metabolites associated with liver toxicity. Toxicology, 441: 152493. https://doi.org/10.1016/j.tox.2020.152493   [Google Scholar] PMid:32479839
  20. Park EY, Murakami H, and Matsumura Y (2005). Effects of the addition of amino acids and peptides on lipid oxidation in a powdery model system. Journal of Agricultural and Food Chemistry, 53(21): 8334-8341. https://doi.org/10.1021/jf058063u   [Google Scholar] PMid:16218685
  21. Rao K, Aziz S, Roome T, Razzak A, Sikandar B, Jamali KS, and Shah MR (2018). Gum acacia stabilized silver nanoparticles based nano-cargo for enhanced anti-arthritic potentials of hesperidin in adjuvant induced arthritic rats. Artificial Cells, Nanomedicine, and Biotechnology, 46(sup1): 597-607. https://doi.org/10.1080/21691401.2018.1431653   [Google Scholar] PMid:29381085
  22. Saad EA, El-Gayar HA, EL-Demerdash RS, and Radwan KH (2018). Hepato-toxic risk of gum arabic during adenine-induced renal toxicity prevention. Journal of Applied Pharmaceutical Science, 8(12): 104-111. https://doi.org/10.7324/JAPS.2018.81212   [Google Scholar]
  23. Saad EA, Toson ESA, and Ahmed GM (2015). Cloveor green tea administration antagonizeskhat hepatotoxicity in rats. International Journal of Pharmacy and Pharmaceutical Sciences, 7(6): 72-76.   [Google Scholar]
  24. Vedi M, Kalaiselvan S, Rasool M, and Sabina EP (2013). Protective effects of blue green algae Spirulina fusiformis against galactosamine-induced hepatotoxicity in mice. Asian Journal of Pharmaceutical and Clinical Research, 6(3): 150-154.   [Google Scholar]
  25. Wang CY, Ma FL, Liu JT, Tian JW, and Fu FH (2007). Protective effect of salvianic acid an on acute liver injury induced by carbon tetrachloride in rats. Biological and Pharmaceutical Bulletin, 30(1): 44-47. https://doi.org/10.1248/bpb.30.44   [Google Scholar] PMid:17202657
  26. Wen S, Wang ZH, Zhang CX, Yang Y, and Fan QL (2020). Caspase-3 promotes diabetic kidney disease through gasdermin E-mediated progression to secondary necrosis during apoptosis. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 13: 313-323. https://doi.org/10.2147/DMSO.S242136   [Google Scholar] PMid:32104028 PMCid:PMC7020918
  27. WHO (1974). Evaluations of some pesticide residues in food. World Health Organization, Geneva, Switzerland.   [Google Scholar]
  28. Williams PA and Phillips GO (2021). Gum arabic. In: Phillips GO and Williams PA (Eds.), Handbook of hydrocolloids: 627-652. 3rd Edition, Woodhead Publishing, Sawston, UK. https://doi.org/10.1016/B978-0-12-820104-6.00022-X   [Google Scholar]
  29. Xie J, Liu G, Eden HS, Ai H, and Chen X (2011). Surface-engineered magnetic nanoparticle platforms for cancer imaging and therapy. Accounts of Chemical Research, 44(10): 883-892. https://doi.org/10.1021/ar200044b   [Google Scholar] PMid:21548618 PMCid:PMC3166427
  30. Yavuz A, Tetta C, Ersoy FF, D’intini V, Ratanarat R, De Cal M, and Ronco C (2005). Reviews: Uremic toxins: A new focus on an old subject. Seminars in Dialysis, 18(3): 203-211. https://doi.org/10.1111/j.1525-139X.2005.18313.x   [Google Scholar] PMid:15934967
  31. Yonis WK and Hassan AH (2020). Antioxidant activity of spirulina powder in male rate with adenine–induced chronic renal failure. Medico-Legal Update, 20(4): 1725-1729. https://doi.org/10.37506/mlu.v20i4.2082   [Google Scholar]
  32. Zhang L, Yu F, Cole AJ, Chertok B, David AE, Wang J, and Yang VC (2009). Gum arabic-coated magnetic nanoparticles for potential application in simultaneous magnetic targeting and tumor imaging. The AAPS Journal, 11(4): 693-699. https://doi.org/10.1208/s12248-009-9151-y   [Google Scholar] PMid:19842043 PMCid:PMC2782085